What's S. griseus. S. griseus is a streptomycin producer.
How determined. We constructed small- (2 kb) and large-insert
(10 kb) genomic libraries, and generated about 110,000 sequences
(giving 9.5-fold coverage) from both ends of the genomic clones. The
sequence data were assembled by using
PHRED/PHRAP/CONSED package and
in-house scripts. Sequence gaps were closed by
transcriptional sequencing and primer walking.
Genes were annotated by using
GLIMMER,
BLASTP,
FramePlot,
tRNASCAN-SE
and in-house scripts.
The telomere sequence has separately been cloned, sequenced, and then
incorporated into the assembly.